Human Ischemia Modified Albumin ELISA Kit | IMA elisa kit
Human Ischemia Modified Albumin ELISA Kit
Reactivity
Human
Synonyms
Ischemia Modified Albumin; N/A; Human Ischemia Modified Albumin ELISA Kit; IMA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of IMA. No significant cross-reactivity or interference between IMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IMA and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive or Sandwich
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 mg/ml
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Related Product Information for IMA elisa kit
Principle of the Assay: IMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IMA. Standards or samples are then added to the microtiter plate wells and IMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IMA are added to each well to "sandwich" the IMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMA concentration in each sample is interpolated from this standard curve.
Intended Uses: This IMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This IMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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