Rat Granzymes B ELISA Kit | Gzms-B elisa kit
Rat Granzymes B ELISA Kit
Gene Names
GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Reactivity
Rat
Synonyms
Granzymes B; N/A; Rat Granzymes B ELISA Kit; Gzms-B elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000 pg/mL
Sensitivity
1.0pg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for Gzms-B elisa kit
Intended Uses: This GMB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GMB. This ELISA kit for research use only!
Principle of the Assay: GMB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GMB antibody and an GMB-HRP conjugate. The assay sample and buffer are incubated together with GMB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GMB concentration since GMB from samples and GMB-HRP conjugate compete for the anti-GMB antibody binding site. Since the number of sites is limited, as more sites are occupied by GMB from the sample, fewer sites are left to bind GMB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GMB concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GMB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GMB antibody and an GMB-HRP conjugate. The assay sample and buffer are incubated together with GMB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GMB concentration since GMB from samples and GMB-HRP conjugate compete for the anti-GMB antibody binding site. Since the number of sites is limited, as more sites are occupied by GMB from the sample, fewer sites are left to bind GMB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GMB concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Gzms-B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,716 Da
NCBI Official Full Name
granzyme B
NCBI Official Synonym Full Names
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
NCBI Official Symbol
GZMB
NCBI Official Synonym Symbols
HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
NCBI Protein Information
granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2
UniProt Protein Name
Granzyme B
UniProt Gene Name
GZMB
UniProt Synonym Gene Names
CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
UniProt Entry Name
GRAB_HUMAN
