Mouse Occludin ELISA Kit | OCLN elisa kit

Mouse Occludin ELISA Kit

Cat. Num. AAA16482

• Gene Names: OCLN; BLCPMG; PPP1R115
• Reactivity: Mouse
• Synonyms: Occludin; N/A; Mouse Occludin ELISA Kit; OCLN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of OCLN. No significant cross-reactivity or interference between OCLN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OCLN and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for OCLN elisa kit

Principle of the Assay: OCLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-OCLN antibody and an OCLN-HRP conjugate. The assay sample and buffer are incubated together with OCLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OCLN concentration since OCLN from samples and OCLN-HRP conjugate compete for the anti-OCLN antibody binding site. Since the number of sites is limited, as more sites are occupied by OCLN from the sample, fewer sites are left to bind OCLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OCLN concentration in each sample is interpolated from this standard curve.

Intended Uses: This OCLN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OCLN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for OCLN elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1322282
• NCBI GeneID: 100506658
• Molecular Weight: 8,175 Da
• NCBI Official Full Name: occludin
• NCBI Official Synonym Full Names: occludin
• NCBI Official Symbol: OCLN
• NCBI Official Synonym Symbols: BLCPMG; PPP1R115
• NCBI Protein Information: occludin; tight junction protein occludin; phosphatase 1, regulatory subunit 115
• UniProt Protein Name: Occludin
• UniProt Gene Name: OCLN
• UniProt Entry Name: OCLN_HUMAN