Rat Calprotectin ELISA Kit | CP elisa kit

Rat Calprotectin ELISA Kit

Cat. Num. AAA16522

• Reactivity: Rat
• Synonyms: Calprotectin; N/A; Rat Calprotectin ELISA Kit; CP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CALPRO. No significant cross-reactivity or interference between CALPRO and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALPRO and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Intended Uses: This CP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CP elisa kit

Principle of the Assay: CALPRO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALPRO antibody and an CALPRO-HRP conjugate. The assay sample and buffer are incubated together with CALPRO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALPRO concentration since CALPRO from samples and CALPRO-HRP conjugate compete for the anti-CALPRO antibody binding site. Since the number of sites is limited, as more sites are occupied by CALPRO from the sample, fewer sites are left to bind CALPRO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALPRO concentration in each sample is interpolated from this standard curve.

Intended Uses: This CALPRO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CALPRO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for CP elisa kit

Immunology

NCBI and Uniprot Product Information

• UniProt Accession #: Q99MG9
• NCBI Official Full Name: calprotectin=urinary stone protein band A {N-terminal}, partial