Porcine Prolactin ELISA Kit | PRL elisa kit
Porcine Prolactin ELISA Kit
Reactivity
Porcine
Synonyms
Prolactin; N/A; Porcine Prolactin ELISA Kit; PRL elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of PRL. No significant cross-reactivity or interference between PRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRL and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Sandwich
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for PRL elisa kit
Intended Uses: This PRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine PRL. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PRL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRL. Standards or samples are then added to the microtiter plate wells and PRL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PRL are added to each well to "sandwich" the PRL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRL concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PRL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRL. Standards or samples are then added to the microtiter plate wells and PRL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PRL are added to each well to "sandwich" the PRL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,876 Da
NCBI Official Full Name
Prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
PRL
NCBI Protein Information
prolactin; decidual prolactin
UniProt Protein Name
Prolactin
UniProt Gene Name
PRL
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_HUMAN
