Rat Hepcidin ELISA Kit | Hepc elisa kit

Rat Hepcidin ELISA Kit

Cat. Num. AAA16608

• Gene Names: HAMP; HEPC; PLTR; HFE2B; LEAP1
• Reactivity: Rat
• Synonyms: Hepcidin; N/A; Rat Hepcidin ELISA Kit; Hepc elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of HEPC. No significant cross-reactivity or interference between HEPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HEPC and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Hepc elisa kit

Principle of the Assay: HEPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HEPC antibody and an HEPC-HRP conjugate. The assay sample and buffer are incubated together with HEPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HEPC concentration since HEPC from samples and HEPC-HRP conjugate compete for the anti-HEPC antibody binding site. Since the number of sites is limited, as more sites are occupied by HEPC from the sample, fewer sites are left to bind HEPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HEPC concentration in each sample is interpolated from this standard curve.

Intended Uses: This EPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HEPC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for Hepc elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 10863973
• NCBI GeneID: 57817
• NCBI Accession #: NP_066998.1
• NCBI GenBank Nucleotide #: NM_021175.2
• Molecular Weight: 9,408 Da
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: HAMP
• NCBI Official Synonym Symbols: HEPC; PLTR; HFE2B; LEAP1
• NCBI Protein Information: hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
• UniProt Protein Name: Hepcidin
• UniProt Gene Name: HAMP
• UniProt Synonym Gene Names: HEPC; LEAP1; UNQ487/PRO1003; LEAP-1; PLTR; Hepc25; Hepc20
• UniProt Entry Name: HEPC_HUMAN