Canine Complement 4 ELISA Kit | C4 elisa kit

Canine Complement 4 ELISA Kit

Cat. Num. AAA16903

• Gene Names: C4; C4-2
• Reactivity: Canine
• Synonyms: Complement 4; N/A; Canine Complement 4 ELISA Kit; C4 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of C4. No significant cross-reactivity or interference between C4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C4 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ?g/mL

• Intended Uses: This C4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine C4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for C4 elisa kit

Principle of the Assay: C4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C4 antibody and an C4-HRP conjugate. The assay sample and buffer are incubated together with C4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4 concentration since C4 from samples and C4-HRP conjugate compete for the anti-C4 antibody binding site. Since the number of sites is limited, as more sites are occupied by C4 from the sample, fewer sites are left to bind C4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This C4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine C4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for C4 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 116175422
• NCBI GeneID: 426611
• NCBI Accession #: NP_001070701.1
• NCBI GenBank Nucleotide #: NM_001077233.1
• NCBI Official Full Name: complement 4
• NCBI Official Synonym Full Names: complement 4
• NCBI Official Symbol: C4
• NCBI Official Synonym Symbols: C4-2
• NCBI Protein Information: complement 4; complement C4; MHC-linked complement C4