Monkey soluble Cluster of differentiation 40 ligand ELISA Kit | Scd40l elisa kit
Monkey soluble Cluster of differentiation 40 ligand ELISA Kit
Reactivity
Monkey
Synonyms
soluble Cluster of differentiation 40 ligand; N/A; Monkey soluble Cluster of differentiation 40 ligand ELISA Kit; Scd40l elisa kit
Reactivity
Monkey
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for Scd40l elisa kit
Intended Uses: This sCD4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sCD4. This ELISA kit for research use only!
Principle of the Assay: sCD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sCD4 antibody and an sCD4-HRP conjugate. The assay sample and buffer are incubated together with sCD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD4 concentration since sCD4 from samples and sCD4-HRP conjugate compete for the anti-sCD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD4 from the sample, fewer sites are left to bind sCD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD4 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: sCD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sCD4 antibody and an sCD4-HRP conjugate. The assay sample and buffer are incubated together with sCD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD4 concentration since sCD4 from samples and sCD4-HRP conjugate compete for the anti-sCD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD4 from the sample, fewer sites are left to bind sCD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD4 concentration in each sample is interpolated from this standard curve.
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