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Standard Curve (Sample)

Canine Interleukin 1 Receptor antagonist ELISA Kit | IL1RA elisa kit

Canine Interleukin 1 Receptor antagonist ELISA Kit

Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Reactivity
Canine
Synonyms
Interleukin 1 Receptor antagonist; N/A; Canine Interleukin 1 Receptor antagonist ELISA Kit; IL1RA elisa kit
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For Research Use Only!
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-1RA. No significant cross-reactivity or interference between IL-1RA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-1RA and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for IL1RA elisa kit
Principle of the Assay: IL-1RA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-1RA antibody and an IL-1RA-HRP conjugate. The assay sample and buffer are incubated together with IL-1RA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1RA concentration since IL-1RA from samples and IL-1RA-HRP conjugate compete for the anti-IL-1RA antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1RA from the sample, fewer sites are left to bind IL-1RA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1RA concentration in each sample is interpolated from this standard curve.

Intended Uses: This IL-1RA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-1RA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for IL1RA elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
16,142 Da
NCBI Official Full Name
interleukin 1 receptor antagonist
NCBI Official Synonym Full Names
interleukin 1 receptor antagonist
NCBI Official Symbol
IL1RN
NCBI Official Synonym Symbols
DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
NCBI Protein Information
interleukin-1 receptor antagonist protein; IL1 inhibitor; IL1RN (IL1F3); type II interleukin-1 receptor antagonist; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra)
UniProt Protein Name
Interleukin-1 receptor antagonist protein
UniProt Gene Name
IL1RN
UniProt Synonym Gene Names
IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP
UniProt Entry Name
IL1RA_HUMAN

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Product Notes

The Canine IL1RA il1rn (Catalog #AAA17034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17034 ELISA Kit recognizes Canine IL1RA. It is sometimes possible for the material contained within the vial of "Interleukin 1 Receptor antagonist, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.