Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-29. No significant cross-reactivity or interference between IL-29 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-29 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for IL-29 elisa kit
Principle of the Assay: IL-29 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-29 antibody and an IL-29-HRP conjugate. The assay sample and buffer are incubated together with IL-29-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-29 concentration since IL-29 from samples and IL-29-HRP conjugate compete for the anti-IL-29 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-29 from the sample, fewer sites are left to bind IL-29-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-29 concentration in each sample is interpolated from this standard curve.
Intended Uses: This IL-29 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IL-29. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This IL-29 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IL-29. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for IL-29 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,898 Da
NCBI Official Full Name
interleukin 29
NCBI Official Synonym Full Names
interferon, lambda 1
NCBI Official Symbol
IFNL1
NCBI Official Synonym Symbols
IL29; IL-29
NCBI Protein Information
interferon lambda-1; IFN-lambda-1; interleukin-29; cytokine Zcyto21; interferon-lambda-1; interleukin 29 (interferon, lambda 1)
UniProt Protein Name
Interferon lambda-1
UniProt Gene Name
IFNL1
UniProt Synonym Gene Names
IL29; ZCYTO21; IFN-lambda-1; IL-29
UniProt Entry Name
IFNL1_HUMAN