Porcine Embryonic Stem MESPU30 ELISA Kit | M30 elisa kit

Porcine Embryonic Stem MESPU30 ELISA Kit

Cat. Num. AAA17194

• Reactivity: Porcine
• Synonyms: Embryonic Stem MESPU30; N/A; Porcine Embryonic Stem MESPU30 ELISA Kit; M30 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of MESPU30. No significant cross-reactivity or interference between MESPU30 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MESPU30 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for M30 elisa kit

Principle of the Assay: MESPU30 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MESPU30 antibody and an MESPU30-HRP conjugate. The assay sample and buffer are incubated together with MESPU30-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MESPU30 concentration since MESPU30 from samples and MESPU30-HRP conjugate compete for the anti-MESPU30 antibody binding site. Since the number of sites is limited, as more sites are occupied by MESPU30 from the sample, fewer sites are left to bind MESPU30-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MESPU30 concentration in each sample is interpolated from this standard curve.

Intended Uses: This MESPU30 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MESPU30. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for M30 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 19717569
• Molecular Weight: 14,749 Da
• NCBI Official Full Name: M30, partial
• UniProt Protein Name: M30
• UniProt Gene Name: M30
• UniProt Entry Name: Q8T667_ECHGR