Human Gamma secretase ELISA Kit | Gamma secretase elisa kit
Human Gamma secretase ELISA Kit
Gene Names
GBE1; GBE; APBD; GSD4
Reactivity
Human
Synonyms
Gamma secretase; N/A; Human Gamma secretase ELISA Kit; Gamma secretase elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GSEC. No significant cross-reactivity or interference between GSEC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSEC and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for Gamma secretase elisa kit
Principle of the Assay: GSEC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSEC antibody and an GSEC-HRP conjugate. The assay sample and buffer are incubated together with GSEC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSEC concentration since GSEC from samples and GSEC-HRP conjugate compete for the anti-GSEC antibody binding site. Since the number of sites is limited, as more sites are occupied by GSEC from the sample, fewer sites are left to bind GSEC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSEC concentration in each sample is interpolated from this standard curve.
Intended Uses: This GSEC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GSEC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This GSEC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GSEC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
80,474 Da
NCBI Official Full Name
glucan (1,4-alpha-), branching enzyme 1 (glycogen branching enzyme, Andersen disease, glycogen storage disease type IV), isoform CRA_b
NCBI Official Synonym Full Names
glucan (1,4-alpha-), branching enzyme 1
NCBI Official Symbol
GBE1
NCBI Official Synonym Symbols
GBE; APBD; GSD4
NCBI Protein Information
1,4-alpha-glucan-branching enzyme; brancher enzyme; glycogen branching enzyme; glycogen-branching enzyme; amylo-(1,4 to 1,6) transglucosidase; amylo-(1,4 to 1,6) transglycosylase
UniProt Protein Name
1,4-alpha-glucan-branching enzyme
UniProt Gene Name
GBE1
UniProt Entry Name
GLGB_HUMAN
