Human Betatrophin ELISA Kit | C19orf80 elisa kit
Human Betatrophin ELISA Kit
Reactivity
Human
Synonyms
Betatrophin; N/A; Human Betatrophin ELISA Kit; C19orf80/Betatrophin/Angiopoietin-like protein 8/Lipasin/Hepatocellular carcinoma-associated protein TD26/UNQ599/PRO1185/Angptl8; C19orf80 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of C19orf80. No significant cross-reactivity or interference between C19orf80 and analogues was observed.
Sequence Length
198
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
46.875-3000pg/ml
Sensitivity
28.125pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Related Product Information for C19orf80 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
betatrophin
NCBI Official Synonym Full Names
angiopoietin-like 8
NCBI Official Symbol
Angptl8
NCBI Protein Information
betatrophin