Rat Gonadotropin-Releasing Hormone ELISA Kit | GnRH elisa kit
Rat Gonadotropin-Releasing Hormone ELISA Kit
Reactivity
Rat
Synonyms
Gonadotropin-Releasing Hormone; N/A; Rat Gonadotropin-Releasing Hormone ELISA Kit; GnRH (Gonadotropin Releasing Hormone); GnRH elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of GnRH. No significant cross-reactivity or interference between GnRH and analogues was observed.
Sequence Length
99
Assay Type
Competitive
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for GnRH elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
11,127 Da
NCBI Official Full Name
gonadotropin releasing hormone, partial
UniProt Protein Name
Gonadotropin releasing hormone
UniProt Entry Name
I1TK91_PARMJ
