Porcine Bone Alkaline Phosphatase ELISA Kit | BALP elisa kit
Porcine Bone Alkaline Phosphatase ELISA Kit
Reactivity
Porcine
Synonyms
Bone Alkaline Phosphatase; N/A; Porcine Bone Alkaline Phosphatase ELISA Kit; BALP elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of BALP. No significant cross-reactivity or interference between BALP and analogues was observed.
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for BALP elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
