Human Induced myeloid leukemia cell differentiation protein Mcl-1 ELISA Kit | MCL1 elisa kit
Human Induced myeloid leukemia cell differentiation protein Mcl-1 ELISA Kit
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human
Synonyms
Induced myeloid leukemia cell differentiation protein Mcl-1; N/A; Human Induced myeloid leukemia cell differentiation protein Mcl-1 ELISA Kit; MCL1/BCL2L3/bcl2-L-3/Bcl2-L-3/MGC104264/Bcl-2-like protein 3/Bcl-2-related protein EAT/mcl1/EAT/induced myeloid leukemia cell differentiation protein Mcl-1/Mcl-1/mcl1/EAT/MCL1-ES/MCL1L/MCL1S/MGC1839/myeloid cell leukemia ES/myeloid cell leukemia sequence 1 (BCL2-related)/TM; MCL1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MCL1. No significant cross-reactivity or interference between MCL1 and analogues was observed.
Sequence Length
197
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Related Product Information for MCL1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,662 Da
NCBI Official Full Name
induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
NCBI Official Synonym Full Names
myeloid cell leukemia 1
NCBI Official Symbol
MCL1
NCBI Official Synonym Symbols
TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Gene Name
MCL1
UniProt Synonym Gene Names
BCL2L3; Bcl2-L-3
UniProt Entry Name
MCL1_HUMAN