Human Insulin-Like Growth Factor 1 ELISA Kit | IGF-1 elisa kit

Human Insulin-Like Growth Factor 1 ELISA Kit

Cat. Num. AAA17690

• Gene Names: IGF1; IGF-1; IGF-I
• Reactivity: Human
• Synonyms: Insulin-Like Growth Factor 1; N/A; Human Insulin-Like Growth Factor 1 ELISA Kit; IGF-1/IGF1/IGFI/IGF-I/IGF1A/IGF-IA/IGF-IB/MGF/Somatomedin C; IGF-1 elisa kit

• Reactivity: Human
• Specificity: Specifically recognize IGF-1, no obvious cross reaction with other analogues
• Sequence Length: 154
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for IGF-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IGF-1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti IGF-1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with IGF-1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IGF-1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 118150782
• NCBI GeneID: 281239
• NCBI Accession #: NP_001071296.1
• NCBI GenBank Nucleotide #: NM_001077828.1
• Molecular Weight: 17,066 Da
• NCBI Official Full Name: insulin-like growth factor I preproprotein
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-1; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-I
• UniProt Entry Name: IGF1_BOVIN