Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SEMG2. No significant cross-reactivity or interference between human SEMG2 and analogues was observed.
Sequence Length
582
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
<7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for SEMG2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMG2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,444 Da
NCBI Official Full Name
semenogelin-2
NCBI Official Synonym Full Names
semenogelin II
NCBI Official Symbol
SEMG2
NCBI Official Synonym Symbols
SGII
NCBI Protein Information
semenogelin-2; Semenogelin 2
UniProt Protein Name
Semenogelin-2
UniProt Gene Name
SEMG2
UniProt Synonym Gene Names
SGII
UniProt Entry Name
SEMG2_HUMAN