herpesvirus 1 Thymidine kinase (TK) Recombinant Protein | TK recombinant protein
Recombinant Human herpesvirus 1 Thymidine kinase (TK)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
herpesvirus 1 Thymidine kinase (TK); N/A; Recombinant Human herpesvirus 1 Thymidine kinase (TK); TK recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-376aa; Full Length
Sequence
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
Related Product Information for TK recombinant protein
In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.
References
The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.McKnight S.L.Nucleic Acids Res. 8:5949-5964(1980)
The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.J. Gen. Virol. 69:1531-1574(1988)
The three-dimensional structure of thymidine kinase from herpes simplex virus type 1.Wild K., Bohner T., Aubry A., Folkers G., Schulz G.E.FEBS Lett. 368:289-292(1995)
Crystal structures of the thymidine kinase from herpes simplex virus type-1 in complex with deoxythymidine and ganciclovir.Brown D.G., Visse R., Sandhu G., Davies A., Rizkallah P.J., Melitz C., Summers W.C., Sanderson M.R.Nat. Struct. Biol. 2:876-881(1995)
The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue.Wild K., Bohner T., Folkers G., Schulz G.E.Protein Sci. 6:2097-2106(1997)
Exploring the active site of herpes simplex virus type-1 thymidine kinase by X-ray crystallography of complexes with aciclovir and other ligands.Champness J.N., Bennett M.S., Wien F., Visse R., Summers W.C., Herdewijn P., de Clerq E., Ostrowski T., Jarvest R.L., Sanderson M.R.3.0.CO;2-8>Proteins 32:350-361(1998)
Structure to 1.9-A resolution of a complex with herpes simplex virus type-1 thymidine kinase of a novel, non-substrate inhibitor
X-ray crystallographic comparison with binding of aciclovir.Bennett M.S., Wien F., Champness J.N., Batuwangala T., Rutherford T., Summers W.C., Sun H., Wright G., Sanderson M.R.FEBS Lett. 443:121-125(1999)
Nucleoside binding site of herpes simplex type 1 thymidine kinase analyzed by X-ray crystallography.Vogt J., Perozzo R., Pautsch A., Prota A., Schelling P., Pilger B., Folkers G., Scapozza L., Schulz G.E.3.0.CO;2-8>Proteins 41:545-553(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45 kDa
NCBI Official Full Name
thymidine kinase
NCBI Official Symbol
UL23
NCBI Protein Information
involved in nucleotide metabolism; thymidine kinase
UniProt Protein Name
Thymidine kinase
UniProt Gene Name
TK
UniProt Entry Name
KITH_HHV11
