Rat Acetyl-Coa Carboxylase (ACC) ELISA Kit | ACC elisa kit
Rat Acetyl-Coa Carboxylase (ACC) ELISA Kit
Reactivity
Rat
Synonyms
Acetyl-Coa Carboxylase (ACC); N/A; Rat Acetyl-Coa Carboxylase (ACC) ELISA Kit; ACC elisa kit
Reactivity
Rat
Sequence Length
146
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 40ng/ml
Sensitivity
0.03ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Standard Curve (Sample)
Related Product Information for ACC elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Acetyl-Coa Carboxylase (also known as ACC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ACC antibody. ACC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ACC Antibody is added and binds to ACC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ACC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ACC antibody. ACC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ACC Antibody is added and binds to ACC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ACC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
16,010 Da
NCBI Official Full Name
acetyl Coa carboxylase
UniProt Protein Name
Biotin carboxyl carrier protein of acetyl-CoA carboxylase
UniProt Gene Name
accB
