Rabbit anti-Human Desmoglein 2/DSG2 Polyclonal Antibody | anti-DSG2 antibody

Anti-Desmoglein 2/DSG2 Antibody

Cat. Num. AAA19196

• Gene Names: DSG2; HDGC; CDHF5
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Desmoglein 2/DSG2, Antibody; Anti-Desmoglein 2/DSG2 Antibody; Desmoglein-2; Cadherin family member 5; HDGC; DSG2; CDHF5 ; anti-DSG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1118

• Applicable Applications for anti-DSG2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human); IHC-P (Embedded Section): 0.5-1ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Immunogen: E Coli-derived human Desmoglein 2/DSG2 recombinant protein (Position: L24-E1020).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

WB (Western Blot)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

FCM (Flow Cytometry)

FCM (Flow Cytometry)

Related Product Information for anti-DSG2 antibody

Description: Rabbit IgG polyclonal antibody for Desmoglein 2/DSG2 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human.

Background: Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current identification criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.

References

1. Arnemann J, Spurr NK, Magee AI, Buxton RS (Jul 1992). "The human gene (DSG2) coding for HDGC, a second member of the desmoglein subfamily of the desmosomal cadherins, is, like DSG1 coding for desmoglein DGI, assigned to chromosome 18". Genomics 13 (2): 484-6.
2. "Entrez Gene: DSG2 desmoglein 2". mutations in DSG2 contribute to the development of ARVD/C.
3. Syrris, P., Ward, D., Asimaki, A., Evans, A., Sen-Chowdhry, S., Hughes, S. E., McKenna, W. J. Desmoglein-2 mutations in arrhythmogenic right ventricular cardiomyopathy: a genotype-phenotype characterization of familial disease. Europ. Heart J. 28: 581-588, 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 116534898
• NCBI GeneID: 1829
• NCBI Accession #: NP_001934.2
• NCBI GenBank Nucleotide #: NP_001934.2
• UniProt Accession #: Q14126
• NCBI Official Full Name: desmoglein-2 preproprotein
• NCBI Official Synonym Full Names: desmoglein 2
• NCBI Official Symbol: DSG2
• NCBI Official Synonym Symbols: HDGC; CDHF5
• NCBI Protein Information: desmoglein-2
• UniProt Protein Name: Desmoglein-2
• UniProt Gene Name: DSG2
• UniProt Synonym Gene Names: CDHF5
• UniProt Entry Name: DSG2_HUMAN