Rabbit FOXK1/MNF Polyclonal Antibody | anti-FOXK1 antibody

Anti-FOXK1/MNF Antibody

Cat. Num. AAA19209

• Gene Names: FOXK1; FOXK1L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: FOXK1/MNF, Antibody; Anti-FOXK1/MNF Antibody; Forkhead box protein F1; Forkhead-related activator 1; FREAC-1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FOXF1; FKHL5; FREAC1; anti-FOXK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 733

• Applicable Applications for anti-FOXK1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml (Human, Mouse, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human FOXK1/MNF recombinant protein (Position: Q175-E671).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

WB (Western Blot)

(Figure 1. Western blot analysis of FOXK1 using anti-FOXK1 antibody.)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

IF (Immunofluorescence)

FCM (Flow Cytometry)

Related Product Information for anti-FOXK1 antibody

Description: Rabbit IgG polyclonal antibody for FOXK1/MNF detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.

Background: Forkhead box protein K1 is a transcription factor of the forkhead box family that in humans is encoded by the FOXK1 gene. It is mapped to 7p22.1. FOXK1 is one of the transcription factors managing the passage from the normal cellular respiration (complete glucose oxidation) to generating ATP and intermediaries for many other biochemical pathways. FOXK1 and its closely relate sibling FOXK2 induce aerobic glycolysis by upregulating the enzymatic machinery required for this (for example, hexokinase-2, phosphofructokinase, pyruvate kinase, and lactate dehydrogenase), while at the same time suppressing further oxidation of pyruvate in the mitochondria by increasing the activity of pyruvate dehydrogenase kinases 1 and 4.

References

1. awke, T. J., Jiang, N., Garry, D. J. Absence of p21(CIP) rescues myogenic progenitor cell proliferative and regenerative capacity in Foxk1 null mice. J. Biol. Chem. 278: 4015-4020, 2003.
2. Huang, J. T.-J., Lee, V. Identification and characterization of a novel human FOXK1 gene in silico. Int. J. Oncol. 25: 751-757, 2004.
3. Katoh, M., Katoh, M. Identification and characterization of human FOXK1 gene in silico. Int. J. Molec. Med. 14: 127-132, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 82546824
• NCBI GeneID: 221937
• NCBI Accession #: NP_001032242.1
• NCBI GenBank Nucleotide #: NP_001032242.1
• UniProt Accession #: P85037
• NCBI Official Full Name: forkhead box protein K1
• NCBI Official Synonym Full Names: forkhead box K1
• NCBI Official Symbol: FOXK1
• NCBI Official Synonym Symbols: FOXK1L
• NCBI Protein Information: forkhead box protein K1
• UniProt Protein Name: Forkhead box protein K1
• UniProt Gene Name: FOXK1
• UniProt Synonym Gene Names: MNF; MNF
• UniProt Entry Name: FOXK1_HUMAN