Rabbit PVRL1/NECTIN1 Polyclonal Antibody | anti-NECTIN1 antibody

Anti-PVRL1/NECTIN1 Antibody

Cat. Num. AAA19303

• Gene Names: PVRL1; ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: PVRL1/NECTIN1, Antibody; Anti-PVRL1/NECTIN1 Antibody; Nectin-1; Herpes virus entry mediator C; Herpesvirus entry mediator C; HveC; Herpesvirus Ig-like receptor; HIgR; Nectin cell adhesion molecule 1; Poliovirus receptor-related protein 1; CD antigen CD111; NECTIN1; HVEC; PRR1; PVRL1; nectin cell adhesion molecule 1; anti-NECTIN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for PVRL1/NECTIN1 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

• Applicable Applications for anti-NECTIN1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml|Human, Mouse, Rat|; IHC-P: 0.5-1ug/ml|Human, Rat|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human PVRL1/NECTIN1 recombinant protein (Position: V32-E237).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-PVRL1/NECTIN1 antibody (AAA19303).Overlay histogram showing PC-3 cells stained with AAA19303 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (AAA19303).PVRL1/NECTIN1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (AAA19303).PVRL1/NECTIN1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (AAA19303).PVRL1/NECTIN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (AAA19303).PVRL1/NECTIN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (AAA19303).PVRL1/NECTIN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (AAA19303).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hek293 whole cell lysatesLane 2: human CCRF-CEM whole cell lysatesLane 3: human Sw620 whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: human SGC-7901 whole cell lysatesLane 6: human Jurkat whole cell lysatesLane 7: rat PC-12 whole cell lysatesLane 8: mouse spleen tissue lysatesLane 9: mouse NIH/3T3 whole cell lysatesLane 10: mouse Raw264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PVRL1/NECTIN1 antigen affinity purified polyclonal antibody (Catalog # AAA19303) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for PVRL1/NECTIN1 at approximately 110KD. The expected band size for PVRL1/NECTIN1 is at 110KD.)

Related Product Information for anti-NECTIN1 antibody

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

References

1. Barron, M. J., Brookes, S. J., Draper, C. E., Garrod, D., Kirkham, J., Shore, R. C., Dixon, M. J. The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice. Hum. Molec. Genet. 17: 3509-3520, 2008.
2. Geraghty, R. J., Krummenacher, C., Cohen, G. H., Eisenberg, R. J., Spear, P. G. Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. Science 280: 1618-1620, 1998.
3. Lopez, M., Eberle, F., Mattei, M. G., Gabert, J., Birg, F., Bardin, F., Maroc, C., Dubreuil, P. Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene. Gene 155: 261-265, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 42560237
• NCBI GeneID: 5818
• NCBI Accession #: NP_002846.3
• NCBI GenBank Nucleotide #: NM_002855.4
• UniProt Accession #: Q15223; O75465; Q2M3D3; Q9HBE6; Q9HBW2
• Molecular Weight: 57,158 Da
• NCBI Official Full Name: poliovirus receptor-related protein 1 isoform 1
• NCBI Official Synonym Full Names: poliovirus receptor-related 1 (herpesvirus entry mediator C)
• NCBI Official Symbol: PVRL1
• NCBI Official Synonym Symbols: ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
• NCBI Protein Information: poliovirus receptor-related protein 1; nectin 1; poliovirus receptor-like 1; herpesvirus Ig-like receptor; herpes virus entry mediator C; ectodermal dysplasia 4 (Margarita Island type)
• UniProt Protein Name: Poliovirus receptor-related protein 1
• UniProt Gene Name: PVRL1
• UniProt Synonym Gene Names: HVEC; PRR1; Herpesvirus entry mediator C; HveC; HIgR
• UniProt Entry Name: PVRL1_HUMAN