Rabbit TCEB2/Elongin-B/ELOB Polyclonal Antibody | anti-ELOB antibody

Anti-TCEB2/Elongin-B/ELOB Antibody Picoband

Cat. Num. AAA19645

• Gene Names: ELOB; SIII; TCEB2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: TCEB2/Elongin-B/ELOB, Antibody; Anti-TCEB2/Elongin-B/ELOB Antibody Picoband; anti-ELOB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-ELOB antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: WB: 0.25-0.5 ug/ml, Human; IHC-P: 2-5 ug/ml, Human, Mouse, Rat; ICC/IF: 5 ug/ml, Human; FC/FACS: 1-3 ug/1x10^6 cells, Human; Direct ELISA: 0.1-0.5 ug/ml, Human; Tested Species: In-house tested species with positive results.; Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P) and ICC.
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human TCEB2/Elongin-B/ELOB recombinant protein (Position: M1-Q118).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 6. Flow Cytometry analysis of JK cells using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).Overlay histogram showing JK cells stained with AAA19645 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCEB2/Elongin-B/ELOB Antibody (AAA19645, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 5. IF analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).TCEB2/Elongin-B/ELOB was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TCEB2/Elongin-B/ELOB Antibody (AAA19645) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).TCEB2/Elongin-B/ELOB was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCEB2/Elongin-B/ELOB Antibody (AAA19645) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).TCEB2/Elongin-B/ELOB was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCEB2/Elongin-B/ELOB Antibody (AAA19645) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).TCEB2/Elongin-B/ELOB was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCEB2/Elongin-B/ELOB Antibody (AAA19645) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCEB2/Elongin-B/ELOB antigen affinity purified polyclonal antibody (#AAA19645) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCEB2/Elongin-B/ELOB at approximately 17 kDa. The expected band size for TCEB2/Elongin-B/ELOB is at 13 kDa.)

Related Product Information for anti-ELOB antibody

Elongin B is a protein that in humans is encoded by the ELOB gene. This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Product Categories/Family for anti-ELOB antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 32699512
• NCBI GeneID: 6923
• UniProt Accession #: Q15370; B7WPD3
• Molecular Weight: 17,911 Da
• NCBI Official Full Name: Elongin-B
• NCBI Official Synonym Full Names: elongin B
• NCBI Official Symbol: ELOB
• NCBI Official Synonym Symbols: SIII; TCEB2
• NCBI Protein Information: elongin-B
• UniProt Protein Name: Elongin-B
• UniProt Gene Name: ELOB
• UniProt Synonym Gene Names: EloB