Rabbit anti-Human SMIM8 Polyclonal Antibody | anti-SMIM8 antibody

Anti-SMIM8 Antibody

Cat. Num. AAA20009

• Gene Names: SMIM8; C6orf162; dJ102H19.2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: SMIM8, Antibody; Anti-SMIM8 Antibody; Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody; anti-SMIM8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Expressed in all tissues.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-SMIM8 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 2-5ug/ml, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human SMIM8 recombinant protein (Position: M1-D97). Human SMIM8 shares 88.7% amino acid (aa) sequence identity with mouse SMIM8.
• Subcellular Localization: Cytoplasm, Nucleus
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross reactivity with other proteins.
• Protein Function: Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

IHC (Immunohistchemistry)

(Figure 9. IHC analysis of SMIM8 using anti-SMIM8 antibody (AAA20009).SMIM8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

Product Categories/Family for anti-SMIM8 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins

References

1. Xutao, D. , Sabino, E. C. , Edecio, C. N. , Ribeiro, A. L. , Barbara, I. , & Charles, M. , et al. (2013). Genome wide association study (gwas) of chagas cardiomyopathy in trypanosoma cruzi seropositive subjects. Plos One, 8(11), e79629.2. The dna sequence and analysis of human chromosome 6. Nature.3. Banks, C. A. S. , Boanca, G. , Lee, Z. T. , Eubanks, C. G. , Hattem, G. L. , & Peak, A. , et al. (2016). Tnip2 is a hub protein in the nf-kappab network with both protein and rna mediated interactions. Molecular & Cellular Proteomics, mcp.M116.060509.

NCBI and Uniprot Product Information

• NCBI GI #: 109948310
• NCBI GeneID: 57150
• NCBI Accession #: NP_001035958.1
• NCBI GenBank Nucleotide #: NM_001042493.2
• UniProt Accession #: Q96KF7; Q5TEZ3; Q6NSD2; Q8IZ10; B2R4V6; E1P505
• Molecular Weight: 11,059 Da
• NCBI Official Full Name: small integral membrane protein 8 isoform 1
• NCBI Official Synonym Full Names: small integral membrane protein 8
• NCBI Official Symbol: SMIM8
• NCBI Official Synonym Symbols: C6orf162; dJ102H19.2
• NCBI Protein Information: small integral membrane protein 8; UPF0708 protein C6orf162
• UniProt Protein Name: Small integral membrane protein 8
• UniProt Gene Name: SMIM8
• UniProt Synonym Gene Names: C6orf162; DC18
• UniProt Entry Name: SMIM8_HUMAN