Bovine Prolactin (PRL) ELISA Kit | PRL elisa kit

Bovine Prolactin (PRL) ELISA Kit

Cat. Num. AAA20596

• Reactivity: Bovine
• Synonyms: Prolactin (PRL); N/A; Bovine Prolactin (PRL) ELISA Kit; PRL elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRL. No significant cross-reactivity or interference between PRL and analogues was observed.
• Sequence Length: 227
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.33ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PRL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PRL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for PRL elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRL in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PRL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PRL elisa kit

Endocrinology; Reproductive science; Genetic science; Hormone metabolism

References

Effects of sodium salicylate and time postpartum on mammary tissue proliferation, gene transcript profile, and DNA methylation; J Dairy Sci; 2021 Oct;104(10):11259-11276.; PMID: 34304880; C M Ylioja; Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.

NCBI and Uniprot Product Information

• NCBI GI #: 254675133
• NCBI GeneID: 5617
• NCBI Accession #: NP_001157030.1
• NCBI GenBank Nucleotide #: NM_001163558.2
• UniProt Accession #: P01236; Q15199; Q92996
• Molecular Weight: 25,876 Da
• NCBI Official Full Name: prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Protein Information: prolactin; decidual prolactin
• UniProt Protein Name: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN