Goat MDH/MDH2 Polyclonal Antibody | anti-MDH2 antibody

MDH/MDH2 Goat anti-Human Polyclonal (Internal Region) Antibody

Cat. Num. AAA21368

• Gene Names: MDH2; MDH; MOR1; M-MDH; MGC:3559
• Reactivity: Mouse, Rat, Pig, Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MDH/MDH2, Antibody; MDH/MDH2 Goat anti-Human Polyclonal (Internal Region) Antibody; IHC-plus MDH/MDH2 Antibody (Internal Region); MDH2; MDH; M-MDH; MGC:3559; MOR1; anti-MDH2 antibody

• Host: Goat
• Reactivity: Mouse, Rat, Pig, Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-MDH2 antibody: Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)
• Application Notes: IHC-P: 5ug/ml; Peptide-ELISA: 1:8000; WB: 0.1-0.3ug/ml; Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 35kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2). Recommended concentration: 0.1-0.3ug/ml. Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2, Rat NP_112413.2 and Pig NP_001231082.1). Recommended concentration: 0.01-0.03ug/ml.
• Target: Human MDH/MDH2
• Immunogen: Peptide with sequence C-TRDDLFNTNAT, from the internal region of the protein sequence according to NP_005909.2.
• Conjugation: Unconjugated
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(MDH/MDH2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry)

(MDH/MDH2 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(MDH/MDH2 Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(MDH/MDH2 Antibody-Antibody (0.1ug/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot)

(MDH/MDH2 Antibody-Antibody (0.01ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot)

(MDH/MDH2 Antibody-Goat anti-MDH2 Antibody (0.1ug/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

WB (Western Blot)

(MDH/MDH2 Antibody-Goat anti-MDH2 Antibody (0.01ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

Related Product Information for anti-MDH2 antibody

MDH2 antibody is an unconjugated goat polyclonal antibody to MDH2 (MDH)(Internal Region) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 21735621
• NCBI GeneID: 4191
• NCBI Accession #: NP_005909.2
• NCBI GenBank Nucleotide #: NM_005918
• UniProt Accession #: P40926; O43682; A8K414; B2RE78; B4DE44; E9PDB2
• NCBI Official Full Name: malate dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: malate dehydrogenase 2, NAD (mitochondrial)
• NCBI Official Symbol: MDH2
• NCBI Official Synonym Symbols: MDH; MOR1; M-MDH; MGC:3559
• NCBI Protein Information: malate dehydrogenase, mitochondrial
• UniProt Protein Name: Malate dehydrogenase, mitochondrial
• UniProt Gene Name: MDH2
• UniProt Entry Name: MDHM_HUMAN