Sheep IL-13 ELISA Kit | IL-13 elisa kit
Sheep IL-13 (Interleukin 13) ELISA Kit
Gene Names
IL13; P600; IL-13
Reactivity
Sheep
Synonyms
IL-13; N/A; Sheep IL-13 (Interleukin 13) ELISA Kit; IL-13 elisa kit
Reactivity
Sheep
Specificity
This kit recognizes natural and some recombinant Sheep IL-13. No Significant cross -reactivity or interference between Sheep IL-13and analogues was observed.
Sequence Length
146
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
7.813-500pg/mL
Sensitivity
Min: 4.688pg/mL; Max: 500pg/mL
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for IL-13 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep IL-13 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-13. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-13. You can calculate the concentration of IL-13 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-13. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-13. You can calculate the concentration of IL-13 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,816 Da
NCBI Official Full Name
interleukin-13
NCBI Official Synonym Full Names
interleukin 13
NCBI Official Symbol
IL13
NCBI Official Synonym Symbols
P600; IL-13
NCBI Protein Information
interleukin-13
UniProt Protein Name
Interleukin-13
UniProt Gene Name
IL13
UniProt Synonym Gene Names
NC30; IL-13
UniProt Entry Name
IL13_HUMAN
