Porcine Peptide YY (PYY) ELISA Kit | PYY elisa kit
Pig Peptide YY (PYY) ELISA Kit
Gene Names
PYY; PYY1; PYY-I
Reactivity
Porcine
Synonyms
Peptide YY (PYY); N/A; Pig Peptide YY (PYY) ELISA Kit; PYY1; Peptide tyrosine tyrosine; PYY elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of PYY. No significant cross-reactivity or interference between PYY and analogues was observed.
Sequence Length
97
Assay Type
Quantitative Competitive
Samples
Porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
< 5.34pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PYY were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PYY were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for PYY elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PYY has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PYY and unlabeled PYY (Standards or samples) with the pre-coated antibody specific to PYY. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PYY in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PYY in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PYY in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PYY in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,360 Da
NCBI Official Full Name
peptide YY preproprotein
NCBI Official Synonym Full Names
peptide YY
NCBI Official Symbol
PYY
NCBI Official Synonym Symbols
PYY1; PYY-I
NCBI Protein Information
peptide YY
UniProt Protein Name
Peptide YY
UniProt Gene Name
PYY
UniProt Synonym Gene Names
PYY
