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WB (Western Blot)
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Granzyme B Recombinant Antibody | GZMB recombinant antibody

Rabbit anti-Granzyme B Recombinant Monoclonal Antibody [BLR022E]

Gene Names
GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified
Synonyms
Granzyme B, Recombinant Antibody; Rabbit anti-Granzyme B Recombinant Monoclonal Antibody [BLR022E]; SECT; fragmentin 2; T-cell serine protease 1-3E; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; HLP; Lymphocyte protease; cytotoxic T-lymphocyte proteinase 2; human lymphocyte protein; CGL1; cytotoxic serine protease B; Fragmentin-2; C11; CCPI; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; cathepsin G-like 1; Granzyme B; GZMB recombinant antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR022E
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for GZMB recombinant antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF)
Application Notes
WB: 1:1000
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
mIF: 1:250
Immunogen
Residues 19-247 (mature protein)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human Granzyme B by western blot. Samples: Whole cell lysate (15 ug) from HEK293T, HeLa, SR, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GranzymeB recombinant monoclonal antibody (AAA23797 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human Granzyme B by western blot. Samples: Myc-tagged Granzyme H and B overexpressing cell lysate (15 ug) prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-myc antibody .)

IHC (Immunohistochemistry)

(Detection of human Granzyme B (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry)

(Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of lymph node. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of human Granzyme B by immunocytochemistry. Sample: FFPE section of Granzyme B over-expressing cells (left) and Granzyme H over-expressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human Granzyme B (shaded) in SR cells by flow cytometry. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for GZMB recombinant antibody
Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing [taken from NCBI Entrez Gene (Gene ID: 3002)]
Product Categories/Family for GZMB recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,716 Da
NCBI Official Full Name
granzyme B
NCBI Official Synonym Full Names
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
NCBI Official Symbol
GZMB
NCBI Official Synonym Symbols
HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
NCBI Protein Information
granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2
UniProt Protein Name
Granzyme B
UniProt Gene Name
GZMB
UniProt Synonym Gene Names
CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
UniProt Entry Name
GRAB_HUMAN

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Product Notes

The GZMB gzmb (Catalog #AAA23797) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Granzyme B Recombinant Monoclonal Antibody [BLR022E] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Granzyme B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF). WB: 1:1000 IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. mIF: 1:250. Researchers should empirically determine the suitability of the GZMB gzmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granzyme B, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.