Mouse Regenerating Islet Derived Protein 3 Beta (REG3b) RTU ELISA Kit | REG3b rtu elisa kit
Mouse Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit
Gene Names
Reg3b; HIP; Pap; PAP1; REG-III
Reactivity
Mouse
Synonyms
Regenerating Islet Derived Protein 3 Beta (REG3b); N/A; Mouse Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit; PAP1; Pancreatitis-Associated Protein 1; REG3b rtu elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of REG3b. No significant cross-reactivity or interference between REG3b and analogues was observed.
Sequence Length
175
Samples
Serum, plasma or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
9.37-600pg/mL
Sensitivity
< 4.1pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG3b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG3b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Standard Curve (Sample)
Related Product Information for REG3b rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of REG3b in mouse serum, plasma or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to REG3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG3b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to REG3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG3b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,476 Da
NCBI Official Full Name
regenerating islet-derived protein 3-beta
NCBI Official Synonym Full Names
regenerating islet-derived 3 beta
NCBI Official Symbol
Reg3b
NCBI Official Synonym Symbols
HIP; Pap; PAP1; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-beta
UniProt Protein Name
Regenerating islet-derived protein 3-beta
UniProt Gene Name
Reg3b
UniProt Synonym Gene Names
Pap; Pap1; REG-3-beta; Reg III-beta
