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Mouse STK33 Monoclonal Antibody | anti-STK33 antibody
STK33 (Serine/Threonine Kinase 33) (AP)
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
STK33, Antibody; STK33 (Serine/Threonine Kinase 33) (AP); Serine/Threonine Kinase 33; anti-STK33 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1F10
Specificity
Recognizes STK33.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
514
Applicable Applications for anti-STK33 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
STK33 (AAH31231, 1aa-514aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEGKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
WB (Western Blot)
(STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in Raw 264.7.)
WB (Western Blot)
(STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in PC-12.)
WB (Western Blot)
(STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in NIH/3T3.)
WB (Western Blot)
(STK33 monoclonal antibody (M09), clone 1F10 Western Blot analysis of STK33 expression in A-431.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-STK33 antibody
Mouse monoclonal antibody raised against a full length recombinant STK33.
Product Categories/Family for anti-STK33 antibody
References
1. STK33 Kinase Activity is Non-Essential in KRAS-Dependent Cancer Cells. Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San Miguel T, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I.Cancer Res. 2011 Jul 8. 2.Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells. Scholl C, Frohling S, Dunn IF, Schinzel AC, Barbie DA, Kim SY, Silver SJ, Tamayo P, Wadlow RC, Ramaswamy S, Dohner K, Bullinger L, Sandy P, Boehm JS, Root DE, Jacks T, Hahn WC, Gilliland DG.Cell. 2009 May 29;137(5):821-34.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Serine/threonine kinase 33
