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Mouse CDK4 Monoclonal Antibody | anti-CDK4 antibody
CDK4 (Cyclin-Dependent Kinase 4, CMM3, MGC14458, PSK-J3) (APC)
Gene Names
CDK4; CMM3; PSK-J3
Applications
Immunofluorescence, Western Blot
Purity
Purified
Synonyms
CDK4, Antibody; CDK4 (Cyclin-Dependent Kinase 4, CMM3, MGC14458, PSK-J3) (APC); Cyclin-Dependent Kinase 4; CMM3; MGC14458; PSK-J3; anti-CDK4 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2G7
Specificity
Recognizes CDK4.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
303
Applicable Applications for anti-CDK4 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
CDK4 (AAH03644, 211aa-303aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
WB (Western Blot)
(CDK4 monoclonal antibody (M04), clone 2G7. Western Blot analysis of CDK4 expression in Raw 264.7.)
WB (Western Blot)
(CDK4 monoclonal antibody (M04), clone 2G7. Western Blot analysis of CDK4 expression in NIH/3T3.)
Application Data
(Detection limit for recombinant GST tagged CDK4 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot)
(CDK4 monoclonal antibody (M04), clone 2G7 Western Blot analysis of CDK4 expression in HeLa.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-CDK4 antibody
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq]
Product Categories/Family for anti-CDK4 antibody
