Rabbit anti-Human Phosphoinositide 3 Kinase, p110 beta Polyclonal Antibody | anti-PI3Kbeta antibody

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Cat. Num. AAA26898

• Gene Names: PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
• Reactivity: Human
• Applications: Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Phosphoinositide 3 Kinase, p110 beta, Antibody; Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol; anti-PI3Kbeta antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the internal domain of human PI3 Kinase, p110 beta. Species Sequence Homology: Monkey, canine, Rat and Mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PI3Kbeta antibody: Immunocytochemistry (ICC), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: ICC: 1:100; ELISA: 0.5ng/well; WB: 0.05-0.1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110 beta (KLH).
• Conjugate: HRP
• Positive Control: HEK-293 cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

WB (Western Blot)

(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

IP (Immunoprecipitation)

(Immunoprecipitation: 10ug of was used to immunoprecipitate from 500ug of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5uL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5ug . Proteins were visualized using anti-rabbit-HRP conjugate and an ECL system.)

ICC (Immunocytochemistry)

(Immunocytochemistry: PI3-Kinase, p110b staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.)

ICC (Immunocytochemistry)

(Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.)

ICC (Immunocytochemistry)

(Confocal Immunocytochemistry Analysis: HeLa cells were fixed, permeablized, and stained with (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor488 (actin, green). Figure on the left has the DAPI filter off.)

ICC (Immunocytochemistry)

(Immunocytochemistry: PI3-Kinase, p110b staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules.)

Product Categories/Family for anti-PI3Kbeta antibody

Antibodies; Protein Kinases

References

1. Krasilnikov, M.A., Biochemistry Mosc. 65(1): 59-67 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 126302584
• NCBI GeneID: 5290
• UniProt Accession #: P42336; Q14CW1; Q99762
• Molecular Weight: 1068
• NCBI Official Full Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
• NCBI Official Symbol: PIK3CA
• NCBI Official Synonym Symbols: MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Gene Name: PIK3CA
• UniProt Synonym Gene Names: PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
• UniProt Entry Name: PK3CA_HUMAN