Rat plasma kallikrein (KLKB1) ELISA Kit | KLKB1 elisa kit

Rat plasma kallikrein (KLKB1) ELISA Kit

Cat. Num. AAA27127

• Gene Names: Klkb1; APS; PSA; Kal3; Klk3; Kal-3
• Reactivity: Rat
• Synonyms: plasma kallikrein (KLKB1); N/A; Rat plasma kallikrein (KLKB1) ELISA Kit; KLKB1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of KLKB1. No significant cross-reactivity or interference between KLKB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KLKB1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for KLKB1 elisa kit

Principle of the Assay: KLKB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KLKB1 antibody and an KLKB1-HRP conjugate. The assay sample and buffer are incubated together with KLKB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLKB1 concentration since KLKB1 from samples and KLKB1-HRP conjugate compete for the anti-KLKB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLKB1 from the sample, fewer sites are left to bind KLKB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLKB1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This KLKB1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat KLKB1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for KLKB1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 236465805
• NCBI GeneID: 16621
• NCBI Accession #: NP_032481.2
• NCBI GenBank Nucleotide #: NM_008455.2
• Molecular Weight: 71,383 Da
• NCBI Official Full Name: plasma kallikrein
• NCBI Official Synonym Full Names: kallikrein B, plasma 1
• NCBI Official Symbol: Klkb1
• NCBI Official Synonym Symbols: APS; PSA; Kal3; Klk3; Kal-3
• NCBI Protein Information: plasma kallikrein; kininogenin; fletcher factor; kallikrein 3, plasma; plasma prekallikrein; antigen, prostate specific
• UniProt Protein Name: Plasma kallikrein
• UniProt Gene Name: Klkb1
• UniProt Synonym Gene Names: Klk3; Pk
• UniProt Entry Name: KLKB1_MOUSE