Bovine Procollagen Type II C-Terminal Propeptide ELISA Kit | P2CP elisa kit
Bovine Procollagen Type II C-Terminal Propeptide ELISA Kit
Reactivity
Bovine
Synonyms
Procollagen Type II C-Terminal Propeptide; N/A; Bovine Procollagen Type II C-Terminal Propeptide ELISA Kit; P2CP elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of PIICP. No significant cross-reactivity or interference between PIICP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PIICP and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for P2CP elisa kit
Principle of the Assay: PIICP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PIICP antibody and an PIICP-HRP conjugate. The assay sample and buffer are incubated together with PIICP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIICP concentration since PIICP from samples and PIICP-HRP conjugate compete for the anti-PIICP antibody binding site. Since the number of sites is limited, as more sites are occupied by PIICP from the sample, fewer sites are left to bind PIICP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIICP concentration in each sample is interpolated from this standard curve.
Intended Uses: This PIICP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PIICP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This PIICP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PIICP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for P2CP elisa kit
