Rat Liver-expressed antimicrobial peptide 2 (LEAP2) ELISA Kit | LEAP2 elisa kit
Rat Liver-expressed antimicrobial peptide 2 (LEAP2) ELISA Kit
Gene Names
LEAP2; LEAP-2
Reactivity
Rat
Synonyms
Liver-expressed antimicrobial peptide 2 (LEAP2); N/A; Rat Liver-expressed antimicrobial peptide 2 (LEAP2) ELISA Kit; LEAP2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of LEAP2. No significant cross-reactivity or interference between LEAP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LEAP2 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for LEAP2 elisa kit
Principle of the Assay: LEAP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LEAP2 antibody and an LEAP2-HRP conjugate. The assay sample and buffer are incubated together with LEAP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LEAP2 concentration since LEAP2 from samples and LEAP2-HRP conjugate compete for the anti-LEAP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LEAP2 from the sample, fewer sites are left to bind LEAP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LEAP2 concentration in each sample is interpolated from this standard curve.
Intended Uses: This LEAP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LEAP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This LEAP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LEAP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for LEAP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,814 Da
NCBI Official Full Name
liver-expressed antimicrobial peptide 2
NCBI Official Synonym Full Names
liver expressed antimicrobial peptide 2
NCBI Official Symbol
LEAP2
NCBI Official Synonym Symbols
LEAP-2
NCBI Protein Information
liver-expressed antimicrobial peptide 2
UniProt Protein Name
Liver-expressed antimicrobial peptide 2
UniProt Gene Name
LEAP2
UniProt Synonym Gene Names
LEAP-2
UniProt Entry Name
LEAP2_HUMAN