Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us
Cart Login
product-image-AAA27265_SC.jpg
Standard Curve (Sample)

Human Acid ceramidase (ASAH1) ELISA Kit | ASAH1 elisa kit

Human Acid ceramidase (ASAH1) ELISA Kit

Gene Names
ASAH1; AC; PHP; ASAH; PHP32; ACDase; SMAPME
Reactivity
Human
Synonyms
Acid ceramidase (ASAH1); N/A; Human Acid ceramidase (ASAH1) ELISA Kit; ASAH1 elisa kit
Ordering
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ASAH1. No significant cross-reactivity or interference between ASAH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASAH1 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ASAH1 elisa kit
Principle of the Assay: ASAH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ASAH1 antibody and an ASAH1-HRP conjugate. The assay sample and buffer are incubated together with ASAH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASAH1 concentration since ASAH1 from samples and ASAH1-HRP conjugate compete for the anti-ASAH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ASAH1 from the sample, fewer sites are left to bind ASAH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASAH1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ASAH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASAH1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
427
Molecular Weight
44,046 Da
NCBI Official Full Name
acid ceramidase
NCBI Official Synonym Full Names
N-acylsphingosine amidohydrolase (acid ceramidase) 1
NCBI Official Symbol
ASAH1
NCBI Official Synonym Symbols
AC; PHP; ASAH; PHP32; ACDase; SMAPME
NCBI Protein Information
acid ceramidase; acid CDase; acylsphingosine deacylase; putative 32 kDa heart protein
UniProt Protein Name
Acid ceramidase
UniProt Gene Name
ASAH1
UniProt Synonym Gene Names
ASAH; HSD-33; HSD33; AC; ACDase; Acid CDase; PHP32
UniProt Entry Name
ASAH1_HUMAN

Similar Products

product-image-AAA19197_WB6.jpg

ASAH1 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA
product-image-AAA27265_SC.jpg

ASAH1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA27266_SC.jpg

ASAH1 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA18758_SDS_PAGE.png

ASAH1 Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a
product-image-AAA16267_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA16403_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA16061_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA17369_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA12896_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA18310_SC.png

CGRP ELISA Kit

Host: n/a
Reactivity: Pig
Apps: n/a
product-image-AAA12955_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA15258_SC.png

CGRP ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA12956_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA11125_SC.jpg

CGRP ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA27257_SC.jpg

ACER1 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA22567_SC.jpg

MPA ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA21040_SC.jpg

BA ELISA Kit

Host: n/a
Reactivity: General
Apps: n/a
product-image-AAA16468_SC.jpg

AA ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA16217_SC.jpg

AA ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA16751_SC.jpg

HA ELISA Kit

Host: n/a
Reactivity: Monkey
Apps: n/a

Product Notes

The Human ASAH1 asah1 (Catalog #AAA27265) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27265 ELISA Kit recognizes Human ASAH1. It is sometimes possible for the material contained within the vial of "Acid ceramidase (ASAH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.