Human Raftlin (RFTN1) ELISA Kit | RFTN1 elisa kit

Human Raftlin (RFTN1) ELISA Kit

Cat. Num. AAA27294

• Gene Names: RFTN1; MIG2; PIG9; PIB10; RAFTLIN
• Reactivity: Human
• Synonyms: Raftlin (RFTN1); N/A; Human Raftlin (RFTN1) ELISA Kit; RFTN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RFTN1. No significant cross-reactivity or interference between RFTN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RFTN1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for RFTN1 elisa kit

Principle of the Assay: RFTN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RFTN1 antibody and an RFTN1-HRP conjugate. The assay sample and buffer are incubated together with RFTN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RFTN1 concentration since RFTN1 from samples and RFTN1-HRP conjugate compete for the anti-RFTN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RFTN1 from the sample, fewer sites are left to bind RFTN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RFTN1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This RFTN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RFTN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 41872577
• NCBI GeneID: 23180
• NCBI Accession #: NP_055965.1
• NCBI GenBank Nucleotide #: NM_015150.1
• Molecular Weight: 63,146 Da
• NCBI Official Full Name: raftlin
• NCBI Official Synonym Full Names: raftlin, lipid raft linker 1
• NCBI Official Symbol: RFTN1
• NCBI Official Synonym Symbols: MIG2; PIG9; PIB10; RAFTLIN
• NCBI Protein Information: raftlin; raft-linking protein; proliferation-inducing protein 9; proliferation-inducing protein 10; cell migration-inducing gene 2 protein
• UniProt Protein Name: Raftlin
• UniProt Gene Name: RFTN1
• UniProt Synonym Gene Names: KIAA0084; MIG2
• UniProt Entry Name: RFTN1_HUMAN