Human cluster of differentiation 15 (CD15) ELISA Kit | CD15 elisa kit
Human cluster of differentiation 15 (CD15) ELISA Kit
Reactivity
Human
Synonyms
cluster of differentiation 15 (CD15); N/A; Human cluster of differentiation 15 (CD15) ELISA Kit; CD15 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CD15. No significant cross-reactivity or interference between CD15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD15 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CD15 elisa kit
Principle of the Assay: CD15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD15 antibody and an CD15-HRP conjugate. The assay sample and buffer are incubated together with CD15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD15 concentration since CD15 from samples and CD15-HRP conjugate compete for the anti-CD15 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD15 from the sample, fewer sites are left to bind CD15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD15 concentration in each sample is interpolated from this standard curve.
Intended Uses: This CD15 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD15. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This CD15 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD15. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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