Monkey Calponin-1 (CNN1) ELISA Kit | CNN1 elisa kit
Monkey Calponin-1 (CNN1) ELISA Kit
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Monkey
Synonyms
Calponin-1 (CNN1); N/A; Monkey Calponin-1 (CNN1) ELISA Kit; CNN1 elisa kit
Reactivity
Monkey
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CNN1 elisa kit
Intended Uses: This CNGA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCNGA3. This ELISA kit for research use only!
Principle of the Assay: CNGA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNGA3 antibody and an CNGA3-HRP conjugate. The assay sample and buffer are incubated together with CNGA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNGA3 concentration since CNGA3 from samples and CNGA3-HRP conjugate compete for the anti-CNGA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNGA3 from the sample, fewer sites are left to bind CNGA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNGA3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CNGA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNGA3 antibody and an CNGA3-HRP conjugate. The assay sample and buffer are incubated together with CNGA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNGA3 concentration since CNGA3 from samples and CNGA3-HRP conjugate compete for the anti-CNGA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNGA3 from the sample, fewer sites are left to bind CNGA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNGA3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CNN1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,170 Da
NCBI Official Full Name
calponin-1
NCBI Official Synonym Full Names
calponin 1, basic, smooth muscle
NCBI Official Symbol
CNN1
NCBI Official Synonym Symbols
SMCC; Sm-Calp; HEL-S-14
NCBI Protein Information
calponin-1; basic calponin; calponins, basic; calponin H1, smooth muscle; epididymis secretory protein Li 14
UniProt Protein Name
Calponin-1
UniProt Gene Name
CNN1
UniProt Entry Name
CNN1_HUMAN
