Rat gamma H2A Histone Family, Member X ELISA Kit | gamma-H2AX elisa kit
Rat gamma H2A Histone Family, Member X ELISA Kit
Reactivity
Rat
Synonyms
gamma H2A Histone Family, Member X; N/A; Rat gamma H2A Histone Family, Member X ELISA Kit; gamma-H2AX elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of H2AFX. No significant cross-reactivity or interference between H2AFX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between H2AFX and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Standard Curve (Sample)
Related Product Information for gamma-H2AX elisa kit
Intended Uses: This H2AFX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat H2AFX. This ELISA kit for research use only!
Principle of the Assay: H2AFX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-H2AFX antibody and an H2AFX-HRP conjugate. The assay sample and buffer are incubated together with H2AFX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the H2AFX concentration since H2AFX from samples and H2AFX-HRP conjugate compete for the anti-H2AFX antibody binding site. Since the number of sites is limited, as more sites are occupied by H2AFX from the sample, fewer sites are left to bind H2AFX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H2AFX concentration in each sample is interpolated from this standard curve.
Principle of the Assay: H2AFX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-H2AFX antibody and an H2AFX-HRP conjugate. The assay sample and buffer are incubated together with H2AFX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the H2AFX concentration since H2AFX from samples and H2AFX-HRP conjugate compete for the anti-H2AFX antibody binding site. Since the number of sites is limited, as more sites are occupied by H2AFX from the sample, fewer sites are left to bind H2AFX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H2AFX concentration in each sample is interpolated from this standard curve.
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