CREB Recombinant Antibody | CREB1 recombinant antibody

Phospho-CREB (Ser133) Antibody, Rabbit MAb

Cat. Num. AAA27785

• Reactivity: Human
• Applications: Functional Assay, Western Blot, Functional Assay, Immunohistochemistry
• Purity: Protein A
• Synonyms: CREB, Recombinant Antibody; Phospho-CREB (Ser133) Antibody, Rabbit MAb; cAMP responsive element binding protein 1; Anti-CREB Antibody; Anti-CREB-1 Antibody; CREB1 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 0049
• Specificity: Reacts with: Human Phospho-CREB (Ser133), may cross with Phospho-ATF-1 (Ser63) by sequence similarity. Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Pig, Goat
• Purity/Purification: Protein A
• Form/Format: Liquid; 10 mM sodium HEPES, 150 mM NaCl, 100ug/mL BSA, 50% glycerol, pH 7.5

• Applicable Applications for CREB1 recombinant antibody: Western Blot (WB)(FAQ Protocol), Immunohistochemistry-Paraffin (IHC-P)(FAQ Protocol)
• Application Notes: WB: 1:10000-1:200000; IHC-P: 1:100-1:500
• Immunogen: A synthetic phosphopeptide corresponding to residues around Ser133 of human CREB.
• Preparation: This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser133 of human CREB.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela treated with TPA (100 nM, 15 min), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:10000, 1:100000, 1:500000 dilution.(Validation Experiment))

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution.(Validation Experiment))

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min)(line B); treated with TPA and lambda-phosphatase (line C) using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution.(Validation Experiment))

IHC (Immunohistochemistry-Paraffin)

(Immunohistochemical analysis of paraffin-embedded human renal carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry-Paraffin)

(Immunohistochemical analysis of paraffin-embedded human lung cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with TPA (200 nM, 15 min; +), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution (upper) or Anti-CREB Antibody, Rabbit Polyclonal at 1:500 dilution (middle) or Beta-Tubulin Loading Control Antibody, Rabbit MAb (Chimeric) at 1:40000 dilution (lower). Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) may cross with Phospho-ATF-1 (Ser63) by sequence similarity.)

Related Product Information for CREB1 recombinant antibody

CAMP responsive element binding protein 1, also known as CREB-1, plays multiple functions as a transcription factor in gene regulation. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. CREB1 proteins are also known to be expressed in several spliced isoforms that act as transcriptional activators or repressors. The activator isoforms, possessing the functional domains for kinase induction and for interaction with other transcriptional regulators, act as transcriptional activators. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB-1 has a complex influence on behavioural responses to drugs of abuse which varies depending on the brain region in which it is expressed. CREB-1 is important for serotonin (5-HT)-induced long-term facilitation (LTF) of the sensorimotor synapse in Aplysia.

Product Categories/Family for CREB1 recombinant antibody

AKT Signaling Pathway

References

Sadamot. H. e. al. (2011. Direc. observatio. o. dimerizatio. betwee. differen. CREB. isoform. i. . livin. cell. PLo. One. . (6). e20285.Li. RY. e. al. (2011. Th. requiremen. fo. enhance. CREB. expressio. i. consolidatio. o. long-ter. synapti. facilitatio. an. long-ter. excitabilit. i. sensor. neuron. o. Aplysia. . Neurosci. 3. (18). 6871-9.Hoeffle. JP. e. al. (1988. Cycli. AMP-responsiv. DNA-bindin. protein. structur. base. o. . clone. placenta. cDNA. Science. 24. (4884). 1430-3.