Reactivity
General
Samples
Animal feeds, Enzyme preparations, Bread improver mixtures and other materials Samples
Detection Range
0.01 mmol/L - 1 mmol/L
Applicable Applications for Basic Xylanase assay kit
Functional Assay
Intra-assay Precision
Intra-assay CV(%) is less than 10%.
Inter-assay Precision
Inter-assay CV(%) is less than 15%.
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Related Product Information for Basic Xylanase assay kit
Background: Xylanase (EC 3.2.1.8) is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4-xylan into xylose, thus breaking down hemicellulose, one of the major components of plant cell walls. As such, it plays a major role in micro-organisms thriving on plant sources for the degradation of plant matter into usable nutrients. Xylanases are produced by fungi, bacteria, yeast, marine algae, protozoans, snails, crustaceans, insect, seeds, etc., (mammals do not produce xylanases). Basic Xylanase Microplate Assay Kit provides a convenient tool for sensitive detection of Basic Xylanase activity in a variety of samples. Xylan is broken down into xylose by xylanase. Xylose is oxidised by NAD+ to D-xylonic acid in the presence of xylose dehydrogenase. The xylanase activity is measured by the increase in absorbance at 450 nm.