Rabbit FUS Polyclonal Antibody | anti-FUS antibody

FUS Polyclonal Antibody

Cat. Num. AAA28170

• Gene Names: FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: FUS, Antibody; FUS Polyclonal Antibody; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2; anti-FUS antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 526

• Applicable Applications for anti-FUS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:100; IF: 1:50 - 1:100
• Species: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_00495.1).
• Preparation and Storage: Store at -20 degree. Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using FUS antibody (AAA28170) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using FUS antibody (AAA28170) at dilution of1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded mouse spleen using FUS antibody (AAA28170) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse brain using FUS antibody (AAA28170) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human breast cancer using FUS antibody (AAA28170) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat brain using FUS antibody (AAA28170) at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody (AAA28170) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% non fat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

WB (Western Blot)

(Western blot analysis of extracts ofvarious cell lines, using FUS antibody (AAA28170) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Related Product Information for anti-FUS antibody

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

Product Categories/Family for anti-FUS antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 544357
• NCBI GeneID: 2521
• UniProt Accession #: P35637; Q9H4A8
• Molecular Weight: Calculated MW: 53kDa; Observed MW: 70kDa
• NCBI Official Full Name: RNA-binding protein FUS
• NCBI Official Synonym Full Names: FUS RNA binding protein
• NCBI Official Symbol: FUS
• NCBI Official Synonym Symbols: TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• NCBI Protein Information: RNA-binding protein FUS; oncogene FUS; oncogene TLS; fus-like protein; fused in sarcoma; 75 kDa DNA-pairing protein; fusion gene in myxoid liposarcoma; translocated in liposarcoma protein; heterogeneous nuclear ribonucleoprotein P2
• UniProt Protein Name: RNA-binding protein FUS
• UniProt Gene Name: FUS
• UniProt Synonym Gene Names: TLS
• UniProt Entry Name: FUS_HUMAN