Rabbit anti-Human, Mouse CASP3 Polyclonal Antibody | anti-CASP3 antibody

CASP3 Polyclonal Antibody

Cat. Num. AAA28248

• Gene Names: CASP3; CPP32; SCA-1; CPP32B
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: CASP3, Antibody; CASP3 Polyclonal Antibody; CPP32; CPP32B; SCA-1; anti-CASP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 277

• Applicable Applications for anti-CASP3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human CASP3
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of Jurkat cells using CASP3 antibody at dilution of 1:100. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours (left). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human breast cancer using CASP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat lung using CASP3 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of Jurkat cells, using CASP3 antibody at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

Related Product Information for anti-CASP3 antibody

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Product Categories/Family for anti-CASP3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 77416852
• NCBI GeneID: 836
• UniProt Accession #: P42574; Q96AN1; Q96KP2; A8K5M2; D3DP53
• Molecular Weight: 32kDa
• NCBI Official Full Name: Caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: CASP3
• NCBI Official Synonym Symbols: CPP32; SCA-1; CPP32B
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• UniProt Gene Name: CASP3
• UniProt Synonym Gene Names: CPP32; CASP-3; CPP-32; SCA-1