Rabbit anti-Human ATPIF1 Polyclonal Antibody | anti-ATPIF1 antibody

ATPIF1 Antibody (N-term)

Cat. Num. AAA28683

• Gene Names: ATPIF1; IP; ATPI; ATPIP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ATPIF1, Antibody; ATPIF1 Antibody (N-term); ATPase inhibitor; mitochondrial; Inhibitor of F(1)F(o)-ATPase; IF(1); IF1; ATPIF1; ATPI; anti-ATPIF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This ATPIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of human ATPIF1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 8-35
• Sequence Length: 60

• Applicable Applications for anti-ATPIF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Function: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
• Cellular Location: Mitochondrion.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(ATPIF1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ATPIF1 antibody detected the ATPIF1 protein (arrow).)

Related Product Information for anti-ATPIF1 antibody

Background: This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].

Product Categories/Family for anti-ATPIF1 antibody

Metabolism; Signal Transduction

References

Sanchez-Cenizo, L., et al. J. Biol. Chem. 285(33):25308-25313(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Li, N., et al. Int. J. Neurosci. 120(3):217-221(2010)
Contessi, S., et al. J. Bioenerg. Biomembr. 39(4):291-300(2007)
Ma, J., et al. Atherosclerosis 191(1):63-72(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 7705927
• NCBI GeneID: 93974
• NCBI Accession #: NP_057395.1; NP_835497.1; NP_835498.1
• NCBI GenBank Nucleotide #: NM_016311.4
• UniProt Accession #: Q9UII2; Q5JXL8; Q6IAQ7; Q9BSL9
• Molecular Weight: Calculated MW: 12249
• NCBI Official Full Name: ATPase inhibitor, mitochondrial isoform 1
• NCBI Official Synonym Full Names: ATPase inhibitory factor 1
• NCBI Official Symbol: ATPIF1
• NCBI Official Synonym Symbols: IP; ATPI; ATPIP
• NCBI Protein Information: ATPase inhibitor, mitochondrial
• UniProt Protein Name: ATPase inhibitor, mitochondrial
• UniProt Gene Name: ATPIF1
• UniProt Synonym Gene Names: ATPI; IF(1); IF1
• UniProt Entry Name: ATIF1_HUMAN