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FCM (Flow Cytometry)
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Rabbit RYR2 Polyclonal Antibody | anti-RYR2 antibody

RYR2 Antibody

Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified.
Synonyms
RYR2, Antibody; RYR2 Antibody; Arrhythmogenic right ventricular dysplasia 2 antibody; ARVC2 antibody; ARVD2 antibody; Brain ryanodine receptor calcium release channel antibody; Brain type ryanodine receptor antibody; Cardiac muscle ryanodine receptor calcium release channel antibody; Cardiac muscle type ryanodine receptor antibody; CCO antibody; Central core disease of muscle antibody; HBRR antibody; hRYR 2 antibody; hRYR2 antibody; MHS antibody; MHS1 antibody; Ryanodine receptor 1 (skeletal) antibody; Ryanodine receptor 1 antibody; Ryanodine receptor 2 (cardiac) antibody; Ryanodine receptor 2 antibody; Ryanodine receptor 3 antibody; Ryanodine receptor type1 antibody; RYDR antibody; RYR 1 antibody; RYR 2 antibody; RYR 3 antibody; RYR antibody; RYR-1 antibody; RyR1 antibody; RYR1_HUMAN antibody; RYR2 antibody; RYR3 antibody; Sarcoplasmic reticulum calcium release channel antibody; Skeletal muscle calcium release channel antibody; Skeletal muscle ryanodine receptor antibody; Skeletal muscle type ryanodine receptor antibody; Skeletal muscle-type ryanodine receptor antibody; SKRR antibody; Type 1 like ryanodine receptor antibody; Type 1 ryanodine receptor antibody; VTSIP antibody; anti-RYR2 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified.
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RYR2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
Synthetic peptide within human RYR2 aa 1450-1550.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining RYR2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining RYR2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining RYR2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.)

DB (Dot Blot)

(Dot blot analysis of anti-RYR2 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR2 antibody was diluted with 1/500.)

Related Product Information for anti-RYR2 antibody
Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca2+ levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
Product Categories/Family for anti-RYR2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
564,567 Da
NCBI Official Full Name
ryanodine receptor 2
NCBI Official Synonym Full Names
ryanodine receptor 2 (cardiac)
NCBI Official Symbol
RYR2
NCBI Official Synonym Symbols
RyR; ARVC2; ARVD2; RYR-2; VTSIP
NCBI Protein Information
ryanodine receptor 2; type 2 ryanodine receptor; islet-type ryanodine receptor; kidney-type ryanodine receptor; cardiac-type ryanodine receptor; cardiac muscle ryanodine receptor-calcium release channel
UniProt Protein Name
Ryanodine receptor 2
UniProt Gene Name
RYR2
UniProt Synonym Gene Names
RYR-2; RyR2; hRYR-2
UniProt Entry Name
RYR2_HUMAN

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Product Notes

The RYR2 ryr2 (Catalog #AAA29945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RYR2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RYR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 ICC: 1:50-1:100 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RYR2 ryr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RYR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.