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FCM (Flow Cytometry)
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Rabbit anti-Human, Rat DKK1 Monoclonal Antibody | anti-DKK1 antibody

DKK1 Antibody

Gene Names
DKK1; SK; DKK-1
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
DKK1, Antibody; DKK1 Antibody; Dickkopf 1 antibody; Dickkopf 1 homolog antibody; Dickkopf 1 like antibody; Dickkopf homolog 1 antibody; Dickkopf like protein 1 antibody; Dickkopf related protein 1 antibody; Dickkopf WNT signaling pathway inhibitor 1 antibody; Dickkopf-1 antibody; Dickkopf-related protein 1 antibody; DKK 1 antibody; Dkk-1 antibody; Dkk1 antibody; DKK1_HUMAN antibody; hDkk 1 antibody; hDkk-1 antibody; SK antibody; anti-DKK1 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
SC06-86
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-DKK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:50-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with DKK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining DKK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DKK1 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of DKK1 on MCF-7 cells lysates using anti-DKK1 antibody at 1/1, 000 dilution.)

Related Product Information for anti-DKK1 antibody
The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3.
Product Categories/Family for anti-DKK1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,672 Da
NCBI Official Full Name
dickkopf-related protein 1
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 1
NCBI Official Symbol
DKK1
NCBI Official Synonym Symbols
SK; DKK-1
NCBI Protein Information
dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1
UniProt Protein Name
Dickkopf-related protein 1
UniProt Gene Name
DKK1
UniProt Synonym Gene Names
Dickkopf-1; Dkk-1; hDkk-1
UniProt Entry Name
DKK1_HUMAN

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Product Notes

The DKK1 dkk1 (Catalog #AAA30134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DKK1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DKK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:50-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the DKK1 dkk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DKK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.