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FCM (Flow Cytometry)
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Rabbit anti-Human DCP1A Monoclonal Antibody | anti-DCP1A antibody

DCP1A Antibody

Gene Names
DCP1A; SMIF; SMAD4IP1; HSA275986; Nbla00360
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
DCP1A, Antibody; DCP1A Antibody; DCP1 decapping enzyme homolog A antibody; Dcp1a antibody; DCP1A_HUMAN antibody; Decapping enzyme hDcp1a antibody; Decapping mRNA 1A antibody; HSA275986 antibody; mRNA decapping enzyme 1A antibody; mRNA-decapping enzyme 1A antibody; Nbla00360 antibody; Putative protein product of Nbla00360 antibody; Smad4 interacting transcriptional co activator antibody; Smad4-interacting transcriptional co-activator antibody; SMAD4IP1 antibody; SMIF antibody; Transcription factor SMIF antibody; anti-DCP1A antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JB51-34
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-DCP1A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of LOVO cells with Dcp1a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining Dcp1a in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Dcp1a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Dcp1a in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.)

Related Product Information for anti-DCP1A antibody
Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs Dcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m (7)GDP, thereby aiding in mRNA degradation. Both Dcp1a and hDcp2 colocalize in the cytoplasm. In addition, Dcp1a interacts with Smad4 forming a complex with TGFbeta and BMP-4. Dcp1a and Smad4 interact directly through a EVH1/WH1 domain on Dcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of Dcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of Dcp1a eliminates TGFbeta-induced nuclear translocation of Dcp1a.
Product Categories/Family for anti-DCP1A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,309 Da
NCBI Official Full Name
mRNA-decapping enzyme 1A
NCBI Official Synonym Full Names
decapping mRNA 1A
NCBI Official Symbol
DCP1A
NCBI Official Synonym Symbols
SMIF; SMAD4IP1; HSA275986; Nbla00360
NCBI Protein Information
mRNA-decapping enzyme 1A; decapping enzyme hDcp1a; transcription factor SMIF; DCP1 decapping enzyme homolog A; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator
UniProt Protein Name
mRNA-decapping enzyme 1A
UniProt Gene Name
DCP1A
UniProt Synonym Gene Names
SMIF
UniProt Entry Name
DCP1A_HUMAN

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product-image-AAA30444_FCM7.jpg

DCP1A Antibody

Host: Rabbit
Reactivity: Human
Apps: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay

Product Notes

The DCP1A dcp1a (Catalog #AAA30444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCP1A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCP1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the DCP1A dcp1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCP1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.